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BU researchers create tool to measure, control protein ...

Oct 20, 2017 · This thread is known as protein aggregation and happens when proteins clump together. These complexes are a hallmark of many diseases, but have recently been linked to beneficial functions as well. Even though protein aggregation is prevalent in biology, many of the causes and consequences are unknown.

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Protein aggregation – Mechanisms, detection, and control ...

Oct 25, 2018 · Protein aggregation has been recognized as one of the major challenges in the development and commercialization of successful protein-based drug products because of the inherent and high aggregation tendency during various manufacturing processes, storage, and administration.

Therapeutic Protein Aggregation: Mechanisms, Design, and ...

A mechanistic view of protein aggregation can help to motivate current and recently developed strategies to control the aggregation processes, and provide context for next-generation approaches based on engineering new proteins or redesigning existing proteins to imbue them with greater aggregation resistance.

Protein Aggregation | Analysis | Measurement

Protein aggregation is a phenomenon that varies widely depending upon the aggregating conditions and the structure of the proteins under observation, with different conformations exhibiting soluble or insoluble, and covalent or non-covalent properties.

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TRiC and Huntingtin Protein Aggregation - hopes.stanford.edu

Protein Folding and Molecular Chaperones^. To understand how TRiC is involved in huntingtin protein aggregation, it is useful to first review the players that make proteins in the cell.First, mRNA instructions are made based on the DNA blueprint in the nucleus, or the control center, of the cell.These instructions are then sent out into the cytoplasm of the cell and are read by structures ...

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Cellular Strategies of Protein Quality Control

The complexity of protein quality control and protein homeostasis likely provides robustness to a process that is critical for cell viability. A surprising emerging concept is that quality control is spatially organized into compartments that manage misfolded proteins hierarchically within the cell.

  • Published in: Cold Spring Harbor Perspectives in Biology · 2011Authors: Bryan Chen · Marco Retzlaff · Thomas Roos · Judith FrydmanAffiliation: Stanford UniversityAbout: Amyloidosis · Homeostasis · Protein folding · Proteome · ChaperoneGet Price

Control of Aggregation, Coaggregation, and Liquid Droplet ...

The properties of electrostatic or hydrophobic interactions, solid-like or liquid-like states, and apparently spherical and amorphous structures are simple but valuable criteria that can be used to control protein aggregation and condensation using small additives.

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Protein Aggregates & Stability | PEGS Europe 2019

The Protein Aggregation & Stability conference at PEGS Europe seeks to highlight the importance in prediction of aggregation propensity affecting stability of solutions and formulations, while exploring new and advanced technologies, strategies and device design to characterize, control and mitigate aggregation.

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Protein Aggregation and Precipitation, Measurement and ...

There are also some general rules for designing mutants with reduced aggregation tendencies. To this end, computational methods may be used to estimate the solvent exposure of residues in 3D‐structures, predict surface patches that may dictate protein–protein interactions, and to model the 3D structure.

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Chapter 9 Protein Aggregation - ETH Zürich

CHAPTER 9. PROTEIN AGGREGATION Figure 9.1: Key di erences between proteins and polymer colloids Figure 9.2: Example of the aggregation behavior of a monoclonal antibody solution at low pH the order of a few nanometers, and are thus lying in the colloidal range. Proteins are therefore

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Protein Aggregation and Precipitation, Measurement and ...

There are also some general rules for designing mutants with reduced aggregation tendencies. To this end, computational methods may be used to estimate the solvent exposure of residues in 3D‐structures, predict surface patches that may dictate protein–protein interactions, and to model the 3D structure.

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Researchers Create Tool to Measure, Control Protein ...

Oct 22, 2017 · yTRAP can be used to study various protein aggregation processes in disease-relevant proteins, RNA-binding proteins and prions.The tool is made of 2 parts – one piece connects to the protein of interest and another part generates of fluorescent signal to measure the quantity of aggregation in a cell.

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ATP-Dependent Dynamic Protein Aggregation Regulates ...

Nov 21, 2018 · In this work, Pu et al. introduced a concept of "dormancy depth" that provides a unifying framework for understanding both persisters and viable but non-culturable cells. Subsequent mechanistic investigations revealed how ATP-dependent dynamic protein aggregation regulates cellular dormancy and resuscitation, the fine control of which facilitates bacterial drug tolerance.

Protein Aggregation | Analysis | Measurement

Protein aggregation is a phenomenon that varies widely depending upon the aggregating conditions and the structure of the proteins under observation, with different conformations exhibiting soluble or insoluble, and covalent or non-covalent properties.

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(PDF) Identification of cell culture conditions to control ...

All shake flask experiments were performed in triplicate and plements reported to control protein aggregation are presented in operated in fed batch mode with daily feeding of a concentrated feed supplement to Table 1B. maintain concentrations of glucose and other nutrients.

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PROTEOSTAT® Protein aggregation standards - ENZ-51039 ...

The PROTEOSTAT ® Protein Aggregation standards are ideal for reliable and accurate quantification of protein aggregation in solution. The standards are used to determine low levels of aggregated protein in a sample by comparing the assay response of a test sample to that of the standard curve; comprised of standards with known concentrations of aggregated IgG.

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Mechanisms of Protein Aggregation : Models to Medicine ...

Biotherapeutics, many of which are proteins, can also aggregate upon long-term storage or stress (e.g., heat, oxidation), leading to loss of efficacy or even undesired immune responses. A better understanding of the underlying features that influence, control, and prevent protein aggregation is essential to improve and protect human health.

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Cellular Strategies of Protein Quality Control

The complexity of protein quality control and protein homeostasis likely provides robustness to a process that is critical for cell viability. A surprising emerging concept is that quality control is spatially organized into compartments that manage misfolded proteins hierarchically within the cell.

  • Published in: Cold Spring Harbor Perspectives in Biology · 2011Authors: Bryan Chen · Marco Retzlaff · Thomas Roos · Judith FrydmanAffiliation: Stanford UniversityAbout: Amyloidosis · Homeostasis · Protein folding · Proteome · ChaperoneGet Price

Protein Aggregation and Stability in Biopharmaceutical ...

The phenomenon of protein aggregation is a complex conundrum that impacts biopharmaceutical development at virtually every stage. All mechanisms of aggregation are not conclusively known, but the industry must use every effort to characterize and control these conditions, applying a rapidly changing landscape of assays, instrumentation, formulation strategies and process steps.

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Hsp70–Bag3 complex is a hub for proteotoxicity-induced ...

Jul 24, 2018 · An unexpected discovery here was that aggresome formation could be regulated at the level of initial protein aggregation. The concept that protein aggregation is controlled by signaling pathways is counterintuitive, but here we identified signaling elements that regulate this process.

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Challenges of Protein Aggregation during Purification ...

"Shear can cause protein aggregation directly or indirectly via cell lysis, which leads to aggregation catalyzed by enzymatic reactions," explains Vedantham. He adds that exposure of proteins at the air-liquid interface during centrifugation and filtration can also .

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